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Features
The Photoconductive Stimulation Device features.
Representation of the LED with stimulation properties depicted.
Feature List
- Hippocampal/Cortical cultures
are optimized for growth on silicon wafers
- The photoconductive effect
alters the conductivity of silicon with exposure to light
- Illuminate as small an area as
approximately 50 microns
- Stimulation protocols can be
from 0.1 Hz up to 100 Hz and approximately up to a 17 volt pulse across
entire surface of wafer
- Only illuminated neuron(s)
depolarize on-demand
- Activity can be detected by
fluo4 or voltage sensitive dyes
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- Study cellular response by
standard protocols or live imaging
- Non-invasive, preserves
cytoskeleton, physiologically benign
- Cell specific (demonstrated by
FM loading)
- Concurrent
electrophysiological recordings demonstrate high frequency stimulation
possible
- Stimulation can result in
controlled synaptic and network remodeling
- Networks formed have complex
activity patterns
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